Coding

Part:BBa_K2247009:Design

Designed by: Jianing Han   Group: iGEM17_Tsinghua   (2017-10-23)


BD-ScFv2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156


Design Notes

None


Source

Constructed from antiAFB1-ScFv2 and yeast Gal4 activator

References

1. Li, X., Li,P. and Zhang,Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influence antibody affinity. Chinese Academy of Agricultural Sciences, 2011.

2. Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.

3. Li, X., Li,P. and Zhang,Q., Molecular characterization of monoclonal antibodies against aflatoxins: a possible explanation for the highest sensitivity. Chinese Academy of Agricultural Sciences, 2011.